Coding

Part:BBa_K1845001:Design

Designed by: Edoardo Gianni   Group: iGEM15_London_Biohackspace   (2015-06-18)


Miraculin (Yeast codon optimised + His-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- Yeast (S. cerevesiae) codon optimized with Genome Compiler - His-tag added - No restriction sites were initially present in the sequence - UTRs removed from the synthesized sequence


Source

Synthesised via IDT gBlocks, sequence taken from GenBank: D38598.1

References